CytospinMouse models of COPD

Mouse model of cigarette smoke-induced inflammation

Features:

  • Infiltration of macrophages and neutrophils in the lung,
  • Cytokine production and growth factor release.

We have previously shown efficacy of anticholinergics, in particular M3 selective anticholinergics, in this model.

Mouse model of elastase-induced emphysema

Features:

  • Increase in mean linear intercept – emphysema,
  • Cytokine production in the lung,
  • Decrease in specific epithelial and endothelial cell markers.
Previous publications

–        Leus NG, van den Bosch T, van der Wouden PE, Krist K, Ourailidou ME, Eleftheriadis N, Kistemaker LE, Bos S, Gjaltema RA, Mekonnen SA, Bischoff R, Gosens R, Haisma HJ, Dekker FJ. HDAC1-3 Inhibitor MS-275 Enhances IL10 Expression in RAW264.7 Macrophages and Reduces Cigarette Smoke-Induced Airway Inflammation in Mice. Sci Rep. 2017 Mar 27;7:45047.

–        Kistemaker LEM, Bos IST, Hylkema MN, Nawijn MC, Hiemstra PS, Wess J, Meurs H, Kerstjens HAM, Gosens R. Muscarinic receptor subtype-specific effects on cigarette smoke-induced inflammation in mice. Eur Respir J 2013; 42:1677-88.

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