Entries by L.E.M Kistemaker

The PDE4 inhibitor CHF-6001 and LAMAs inhibit bronchoconstriction-induced remodeling in lung slices

Kistemaker LEM, Oenema TA, Baarsma HA, Bos IST, Schmidt M, Facchinetti F, Civelli M, Villetti G, Gosens R. Am J Physiol Lung Cell Mol Physiol. 2017 Sep 1;313(3):L507-L515. Publication: http://www.ncbi.nlm.nih.gov/pubmed/28596292 Abstract Combination therapy of PDE4 inhibitors and anticholinergics induces bronchoprotection in COPD. Mechanical forces that arise during bronchoconstriction may contribute to airway remodeling. Therefore, we investigated […]

HDAC-3 selective inhibitor RGFP966 demonstrates anti-inflammatory properties by attenuating NF-κB p65 transcriptional activity

Leus NG, van der Wouden PE, van den Bosch T, Hooghiemstra WT, Ourailidou ME, Kistemaker LEM, Bischoff R, Gosens R, Haisma HJ & Dekker FJ. Biochem Pharmacol. 2016 May 15;108:58-74. Publication: http://www.ncbi.nlm.nih.gov/pubmed/26993378 Abstract The increasing number of patients suffering from chronic obstructive pulmonary disease (COPD) represents a major and increasing health problem. Therefore, novel therapeutic approaches […]

Combination therapy of tiotropium and ciclesonide attenuates airway inflammation and remodeling in a guinea pig model of chronic asthma

Kistemaker LEM, Bos IST, Menzen MH, Maarsingh H, Meurs H & Gosens R. Resp Res 2016 Feb 4. Publication: http://www.ncbi.nlm.nih.gov/pubmed/26846267 Abstract Background: The long-acting anticholinergic tiotropium has recently been registered for the treatment of asthma, and its use is associated with a reduction in exacerbation frequency. Anti-inflammatory and anti-remodeling effects of tiotropium have been demonstrated in […]

Anti-inflammatory effects of targeted lung denervation in patients with COPD.

Kistemaker LEM, Slebos DJ, Meurs H, Kerstjens HAM & Gosens R. Eur Respir J. 2015 Sep 4. Publication: http://www.ncbi.nlm.nih.gov/pubmed/26341988 Abstract Background: Acetylcholine does not only induce bronchoconstriction, but is also identified as a regulator of airway inflammation in animal models of COPD. This has not been demonstrated in patients with COPD. Targeted lung denervation (TLD) is a […]

Tiotropium attenuates IL-13-induced goblet cell metaplasia of human airway epithelial cells.

Kistemaker LEM, Hiemstra PS, Bos IST, Bouwman S, van den Berge M, Hylkema MN, Meurs H, Kerstjens HAM & Gosens R. Thorax, 2015, Jul;70(7):668-76. Publication: http://www.ncbi.nlm.nih.gov/pubmed/25995156 Abstract Background: It has been shown that acetylcholine is both a neurotransmitter and acts as a local mediator, produced by airway cells including epithelial cells. In vivo studies have demonstrated an […]

Acetylcholine beyond bronchoconstriction: roles in inflammation and remodeling.

Kistemaker LEM & Gosens R. Trends Pharmacol Sci., 2015 Mar;36(3):164-71.  Publication: http://www.ncbi.nlm.nih.gov/pubmed/25511176 Abstract Acetylcholine is the primary parasympathetic neurotransmitter in the airways, where it not only induces bronchoconstriction and mucus secretion, but also regulates airway inflammation and remodeling. In this review, we propose that these effects are all primarily mediated via the muscarinic M3 receptor. Acetylcholine […]

Muscarinic M3 receptors contribute to allergen-induced airway remodeling in mice.

Kistemaker LEM, Bos IST, Hylkema MN, Hiemstra PS, Wess J, Meurs H, Kerstjens HAM & Gosens R. Am J Respir Cell Mol Biol., 2014 Apr;50(4):690-8. Publication: http://www.ncbi.nlm.nih.gov/pubmed/24156289 Abstract Rationale: Asthma is a chronic obstructive airway disease, characterized by inflammation and remodeling. Acetylcholine contributes to symptoms by inducing bronchoconstriction via the muscarinic M3 receptor. Recent evidence suggests that bronchoconstriction […]

Muscarinic receptor subtype-specific effects on cigarette smoke-induced inflammation in mice.

Kistemaker LEM, Bos IS, Hylkema MN, Nawijn MC, Hiemstra PS, Wess J, Meurs H, Kerstjens HAM, Gosens R. Eur Respir J., 2013, Dec;42(6):1677-88. Publication: http://www.ncbi.nlm.nih.gov/pubmed/23397297 Abstract Cholinergic tone contributes to airflow obstruction in chronic obstructive pulmonary disease. Accordingly, anticholinergics are effective bronchodilators by blocking the muscarinic M3 receptor on airway smooth muscle. Recent evidence indicates that acetylcholine also contributes to airway inflammation. However, which muscarinic […]